Coding

Part:BBa_K3190106

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-13)


Homo sapiens luteinizing hormone/choriogonadotropin receptor (LHCGR), CDS with Linker-superfolded GF

The luteinizing hormone/choriogonadotropin receptor (LHCGR), also lutropin/choriogonadotropin receptor (LCGR) or luteinizing hormone receptor (LHR) is a transmembrane receptor found predominantly in the ovary and testis. The gene for the LHCGR is found on chromosome 2 p21 in humans, close to the FSH receptor gene. The coding sequence for the receptor Hu-LHCGR was codon optimised and fused with the nucleotides for the linker and superfolded GFP in the C-terminus (Hu-LHCGR-Li-sfGFP) and coupled to the strongest constitutive promoter pCCW12 for heterologous expression in S. cerevisiae. The construct was important to carry out colocalisation assay and characterise the expression and proper alignment of the receptor in the intercellular organelles.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1643
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 145
    Illegal NgoMIV site found at 1324
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1558
    Illegal SapI.rc site found at 2134


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Categories
Parameters
None